导师姓名：荆晓艳
出生年月：1983.08
职称：特聘四级教授
学历：博士
导师类别：硕士生导师
招生方向：功能微生物资源挖掘、绿色低碳益生菌剂的研发和质检
联系方式： jingxy@qibebt.ac.cn
一、个人简介
面向国家绿色低碳高质量发展的重大需求，聚焦“绿色环境功能微生物的资源挖掘和益生菌剂开发及应用”为导向的研究工作，在不依赖于培养的绿色环境种质筛选技术研究中，成功建立了耦合拉曼分选的环境微生物“代谢表型组-基因组”方法学平台；首次实现了精确到一个细菌细胞、全基因组覆盖度超过92%的耦合功能分选的环境微生物单细胞精度解析；发明“先筛后养”的单细胞拉曼耦合培养等技术；开发“物种-代谢”双靶向的微生物细胞及酶资源挖掘新技术等。基于这些技术突破，正不断建立多类单细胞水平的、基于生化表型的新型细胞种质筛选体系。在环境益生菌群单细胞精度的功能分析方法方面的相关研究成果已发表在The Innovation、ISME、ISME Communications、Environmental Microbiology和mSystems等期刊上。
二、研究方向
主要研究兴趣为环境功能微生物单细胞精度的组学解析和培养
三、主要科研项目
1.国家自然科学基金面上项目，基于单细胞拉曼耦合基因组和转录组测序的海洋的海洋近原位微生物组固碳机制解析，2023.01-2026.12，主持；
2.国家重点研发计划项目课题，红壤关键耐逆微生物群的单细胞组学识别与调控；2021.12-2025.12，主持；
3.国家重点研发计划项目子课题，东北地区典型中低产田沃土生物网络构建与多功能性靶向调控技术；2025.01-2028.12，主持；
4.国家自然科学基金青年科学基金项目，基于单细胞拉曼分选耦合精准宏基因组的海洋原位固碳微生物解析，2019.01-2021.12，主持；
5.企业横向项目-青岛星赛生物科技有限公司，环境功能菌筛选领域单细胞分析分选技术服务，2023.10-2024.03，主持；
6.中科院知识创新工程重要方向项目，新一代微生物群落元基因组比较、分析与评价的标准化研究，主持；
7.国家自然科学基金重点项目，单细胞精度的菌群“代谢表型组-基因组”方法学研究；2021.01-2025.12，骨干。
8.中国科学院科研仪器设备研制项目，全自动单细胞拉曼探测与挖掘系统；2024.07-2025.06，骨干。
四、代表性论文：
1.Jing XY, Gong YH, Diao ZD, Ma Y, Meng Y, Chen J, Ren YS, Li YC, Sun WH, Zhang J, Ji YT, Liang YT, Cong ZQ, Li SY, Ma B. Cui ZS, Ma Y, Xu J. Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function by FISH-scRACS-Seq. The Innovation, 100759, 2025.
2.Li Sen, Huang WG, Peng CR, Jing XY, Ding JX, Chen T, Huang RL, Hu H, Zhou JZ, Zhang JB, Liang YT. Enhancement of rice production and soil carbon sequestration utilizing nitrogen-ffxing cyanobacteria. Applied Soil Ecology 207(2025)105941, 2025.
3.Diao ZD, Jing XY, Hou XB, Meng Y, Zhang JP, Wang YS, Ji YT, Ge AL, Wang XX, Lang YT, Xu J, Ma B. Artificial intelligence-assisted automatic Raman-activated cell sorting (AI-RACS) system for mining specific functional microorganisms in the microbiome. Analytical Chemistry, 96(46), 2024.
4.Jing XY, Gong YH, Pan HH, Meng Y, Ren YS, Diao ZD, Mu RZ, Xu T, Zhang J, Ji YT, Li YD, Wang C, Qu LY, Cui L, Ma B, Xu J. Single-cell Raman-activated sorting and cultivation (scRACS-Culture) for assessing and mining in situ phosphate-solubilizing microbes from nature. ISME Communications, 2(1), 2022.
5.Jing XY, Gong YH, Xu T, Meng Y, Han X, Su XL, Wang JM, Ji YT, Li YD, Jia ZJ, Ma B, Xu J. One-cell metabolic phenotyping and sequencing of soil microbiome by Raman-activated gravity-driven encapsulation (RAGE). mSystems, 29;6(3):e0018121, 2021.
6.Jing XY, Gou HL, Gong YH, Ji YT, Su XL, Zhang J, Han MZ, Xu L, Wang TT. Seasonal dynamics of the coastal bacterioplankton at intensive fish-farming areas of the Yellow Sea, China revealed by high-throughput sequencing. Marine Pollution Bulletin, 139:366-375, 2019.
7.Jing XY, Gong YH, Xu T, Paul A. Davison, Craig MacGregor-Chatwin, C. Neil Hunter, Xu L, Meng Y, Ji YT, Ma B, Jian Xu, Huang W. Revealing CO2-fixing SAR11 bacteria in the ocean by Raman-based single-cell metabolic profiling and genomics. BioDesign Research, 2022, 2022.
8.Jing XY, Gou HL, Gong YH, Su XL, Xu L, Ji YT, Ian P. Thompson, Xu J, Huang W. Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean. Environmental Microbiology, 20(6):2241-2255, 2018.
9.Ni HW, Jing XY, Xiao X, Zhang N, Wang XY, Sui YY, Sun B, Liang YT. Microbial metabolism and necromass mediated fertilization effect on soil organic carbon after long-term community incubation in different climates. The ISME Journal, 15:2561–2573, 2021.
10.Cui ZS, Li YC, Jing XY, Luan X, Liu N, Liu JY, Meng Y, Xu J, David L. Valentine. Cycloalkane degradation by an uncultivated novel genus of Gammaproteobacteria derived from China’s marginal seas. Journal of Hazardous Materials, 469:133904, 2024.
11.Diao ZD, Wang XX, Zhang JP, Ge AL, Xu T, Kan LY, Li YD, Ji YT, Jing XY, Xu J, Ma B. Optical-based microbubble for on-demand droplet release from static droplet array (SDA) for dispensing one droplet into one tube. Biosensors and Bioelectronics, 240:115639, 2023.
12.Zhang J, Su XL, Wang YF, Wang XH, Zhou SQ, Jia H, Jing XY, Gong YH, Wang JC, Xu J. Improved single-cell genome amplification by a high-efficiency phi29 DNA polymerase. Frontier in Bioengineering and Biotechnology, 29(11):1233856, 2023.
13.Zhang J, Ren LH, Zhang L, Gong YH, Xu T, Wang XH, Guo C, Zhai L, Yu XJ, Li Y, Zhu PF, Chen RZ, Jing XY, Jing GC, Zhou SQ, Xu MY, Wang C, Niu CK, Ge YY, Ma B, Shang GS, Cui YL, Yao S, Xu J. Single-cell rapid identification, in situ viability and vitality profiling, and genome-based source-tracking for probiotics products. iMeta, doi.org/10.1002/imt2.117, 2023.
14.Zhang J, Xu T, Wang XH, Jing XY, Zhang J, Hong J, Xu J, Wang JC. Lignocellulosic xylitol production from corncob using engineered Kluyveromyces marxianus. Frontiers in Bioengineering and Biotechnology, 10, 2022.
15.Diao ZD; Kan LY; Zhao YL; Yang HB; Song JY; Wang C; Liu Y; Zhang FL; Xu T; Chen RZ; Ji YT; Wang XX; Jing XY; Xu J; Li YD; Ma B. Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube. mLife, doi: 10.1002/mlf2.12047, 2022.
16.Su XL*, Gong YH*, Gou HL, Jing XY, Xu T, Zheng XS, Chen RZ, Li YD, Ji YT, Ma B, Xu J. Rational Optimization of Raman-Activated Cell Ejection and Sequencing for Bacteria. Analytical Chemistry, 92(12), 2020.
17.Wei L, Hajjami ME, Shen C, You WX, Lu YD, Li J, Jing XY, Hu Q, Zhou WX, Poetsch A, Xu J. Transcriptomic and proteomic responses to very low CO2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica. Biotechnology for Biofuels, 168, 2019.
18.Xu CG, Huang RR, Teng L, Jing XY, Hu JQ, Cui GZ, Wang YL, Cui Q, Xu J. Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization. Nature Communications, 6900, 2015.
19.Fan JH, Ning K, Zeng XW, Luo YC, Wang DM, Hu JQ, Li J, Xu H, Huang JK, Wan MX, Wang WL, Zhang DJ, Shen GM, Run CL, Liao JJ, Fang L, Huang S, Jing XY, Su XQ, Wang AH, Bai LL, Hu ZM, Xu J, Li YG (2015). Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.. Plant Physiology, 169(4):2444–2461, 2015.
20.Wang DM, Ning K, Li J, Hu JQ, Han DX, Wang H, Zeng XW, Jing XY, Zhou Q, Su XQ, Chang XZ, Wang AH, Wang W, Jia J, Wei L, Xin Y, Qiao YH, Huang RR, Chen J, Han B, Yoon K, T.Hill R, Zohar Y, Chen F, Hu Q, Xu J. Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits. Plos Genetics, 10(1): e1004094, 2014.
21.Wei L, Xin Y, Wang DM, Jing XY, Zhou Q, Su XQ, Jia J, Ning K, Chen F, Hu Q, Xu J. Nannochloropsis plastid and mitochondrial phylogenomes reveal organelle diversification mechanism and intragenus phylotyping strategy in microalgae. BMC Genomics, 534, 2013.
22.Li J, Han DX, Wang DM, Ning K, Jia J, Wei L, Jing XY, Hua S, Chen J, Li YT, Hu Q, Xu J. Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae. Plant Cell, 26(4):1645-1665, 2014.
23.孙鹏城，潘慧慧，荆玉姝，夏文香，任义尚，荆晓艳*。单细胞视角下污水反硝化聚磷菌资源挖掘与代谢机制的研究进展，微生物学报10.13343/j.cnki.wsxb.20240650，2025。
24.荆晓艳，黄倢，张士璀。核酸检测技术在水产动物病毒感染诊断中的应用，动物医学进展，29(9)：95-99，2008。
25.陈同, 赵远, 彭成荣, 荆晓艳, 梁玉婷。单细胞水平解析土壤固氮鱼腥藻的碳氮固存过程研究进展，微生物学报64(6)：1721-1734，2024。
26.刘梦泽, 崔鸿鹏, 李冰, 宫达, 范晓鹏, 徐健, 荆晓艳, 王琛, 蒋宏忱。东南极达尔克冰川-183m深处冰层融水细菌多样性研究，微生物学报64(6)：1864-1875，2024。
五、专利
1.荆晓艳，潘慧慧，孟宇，籍月彤，徐健；一种富集污水微生物以用于单细胞精度表型测量和分选的试剂盒及方法；2021年；专利授权号：ZL202110510521.1;
2.荆晓艳，潘慧慧，徐健；一种高效聚磷菌及其应用；2024年；申请号：202410916199.6；
3.荆晓艳，潘慧慧，徐健；基于单细胞拉曼光谱技术的微生物聚磷活性评价方法及分选方法；2024年；申请号：202410916257.5；
4.荆晓艳，孟宇，徐健；一种具有铝耐受力的解磷菌及其应用；2024年；申请号：202410916040.4；
5.荆晓艳，徐健，孟宇，张怀志，徐岩；一种针对低细胞量土壤样本的细胞提取方法；2023年；申请号：202311044666.2;
6.勾洪磊，荆晓艳，籍月彤，徐健；一种单细胞弹射基片、方法及应用；2018年；专利授权号：ZL201810041167.0；
7.勾洪磊，荆晓艳，苏晓璐，籍月彤，徐健；一种用于单细胞快速拉曼测量和激光弹射分选的一体化装置；2017年；专利授权号：ZL201710674974.1；
8.徐健，苏晓璐，籍月彤，荆晓艳，公衍海；单细胞全基因组扩增试剂盒及其应用；2019年；专利授权号：ZL201911166909.3;
9.黄倢，荆晓艳，杨冰，张庆利，陈新华，江育林，刘荭，秦启伟，绳秀珍，史成银，宋晓玲，刘莉；检测多种鱼类病原的基因芯片及其检测方法；2010年；专利授权号：ZL2010 1 0243398.3；
10.黄倢，荆晓艳，杨冰，张庆利，闫丽，王秀华，张保存，宋晓玲，史成银，刘莉；检测多种对虾病原的基因芯片及其检测方法；2010年；专利授权号：ZL2010 1 0243399.8。
